- 商品介绍
- 规格参数
- 包装参数
General description
Transcription factor SOX-17 (UniProt: Q9H6I2; also known as SOX-17) is encoded by the SOX17 gene (Gene ID: 64321) in human. SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5′-AACAAT-3 or 5′-AACAAAG-3′ and modulates transcriptional regulation via WNT3A. It is reported to act upstream of the Notch system and downstream of the canonical Wnt system and provides a link between the canonical Wnt and Notch signaling. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues, such as pancreas and liver. Although detected in multiple tissues, it is preferentially expressed in esophagus, stomach and small intestine. SOX17 required for normal looping of the embryonic heart tube and in embryonic and postnatal vascular development. Inactivation of SOX17 in endothelial cells in mouse embryo is reported to cause a lack of arterial differentiation and vascular remodeling resulting in death in utero. Embryonic stem cells that are deficient in SOX17 fail to differentiate into extraembryonic cell types and maintain expression of pluripotency-associated transcription factors. However, forced expression of SOX17 results in down-regulation of embryonic stem cell associated gene expression and activates genes that are involved in differentiation toward an extraembryonic endoderm cell fate. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Corada, M., et al. (2013). Nat. Commun. 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
His-tagged recombinant fragment corresponding to 149 amino acids from the C-terminal region of human SOX-17.
Application
Anti-SOX17, clone 3F7 ZooMAb, Cat. No. ZRB1345, is a recombinant Rabbit monoclonal antibody that specifically targets SOX17 and has been tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant SOX17 with a KD of 5.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects SOX-17. It targets an epitope within the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3F7, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | observed mol wt ~60 kDa,calculated mol wt 44.12 kDa |
| species reactivity | canine, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunofluorescence: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q9H6I2 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SOX17(64321) |
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