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General description
TNF receptor-associated factor 6 (UniProt: Q9Y4K3; also known as EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6) is encoded by the TRAF6 (also known as RNF85) gene (Gene ID: 7189) in human. TRAF6 is a RING-type E3 ligase that plays a vital role in immune signal transduction pathways. It is composed of an N-terminal RING domain followed by at least four zinc finger domains (ZF1-4), a central coiled-coil domain, and a C-terminal TRAF-C domain. It catalyzes lysine 63-linked polyubiquitination using the Ubc13/Uev1A complex as the E2. It participates in many protective responses in immune and non-immune cells. TRAF6 is detected in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Upon receptor activation, TRAF6 is recruited to upstream signal transduction complexes and activates downstream IkB kinase (IKK) or MAP kinases leading to the activation of NF-kB and AP-1 transcription factors. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dainichi, T., et al. (2019). Front. Immunol. 10; 1107; Fu, T-M., et al. (2018). Proc. Natl. Acad. Sci. USA.115(8); 1783-1788).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human TNF receptor-associated factor 6 (TRAF6).
Application
Quality Control Testing
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRAF6 in K562 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TRAF6 in COS-7, C6, HEK293, and U2OS cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound TRAF6 peptide with a KD of 5.7 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TRAF6 in HEK293 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRAF6 in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1M14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects TNF receptor-associated factor 6 (TRAF6). It targets an epitope within 17 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1M14, recombinant monoclonal |
| description | 1M14 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 59.57 kDa,observed mol wt ~58 kDa |
| purified by | using Protein A |
| species reactivity | rat, monkey, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminus |
| Protein ID accession no. | NP_004611 |
| UniProt accession no. | Q9Y4K3 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TRAF6(7189) |
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