- 商品介绍
- 规格参数
- 包装参数
General description
The STR profile of this cell line matches that of its parental cell line ATCC® Catalog No. CRL-1619. A375 are a human malignant melanoma cell line isolated from a 54 year old female. The cells possess a hypotriploid karyotype.
Application
This product is a human A375 melanoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.
Biochem/physiol Actions
These cells are a human malignant melanoma cell line isolated from a 54-year old female. The cells possess a hypotriploid karyotype.
Features and Benefits
These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A375 cells are adherent with a doubling time of approximately 22 hours.
Preparation Note
DMEM (Catalog Number D6429) with 10% FBS (Catalog Number F2442)
Analysis Note
Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.
Legal Information
ATCC is a registered trademark of American Type Culture Collection
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | human female skin (Source Disease: Malignant melanoma) |
| Quality Segment | 200 |
| growth mode | Adherent |
| technique(s) | cell culture | mammalian: suitable |
| web metadata keyword.default | A375 cancer cell line for drug testing,A375 cell line with integrated mKATE2 gene,A375 cells with mKATE2 fluorescence,A375 hypotriploid karyotype cell line,A375 melanoma cell line for research,A375 melanoma cells for lab use,ATCC CRL-1619 A375 cell line,CompoZr Zinc Finger Nuclease A375 cells,EF1a promoter A375 cell line,Sigma-Aldrich A375 cancer cell line,human A375 cell line,human malignant melanoma A375 cell line,landing pad A375 cell line for gene editing,mammalian A375 cell culture products |
| shipped in | dry ice |
| storage temp. | −196°C |
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