Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit,0.5ML,A4062-.5ML,Sigma

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订货号 0BU3394
品牌型号 Sigma A4062-.5ML
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.


Immunogen

purified goat IgG


Application

Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate.
Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) .


Biochem/physiol Actions

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins.


Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide


Preparation Note

Adsorbed to reduce background staining with human samples.


Other Notes

Antibody adsorbed with human serum proteins.


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment200
conjugatealkaline phosphatase conjugate
antibody formaffinity isolated antibody
antibody product typesecondary antibodies
clonepolyclonal
formbuffered aqueous glycerol solution
species reactivitygoat
technique(s)direct ELISA: 1:30,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50,western blot: 1:30,000
shipped inwet ice
storage temp.2-8°C
target post-translational modificationunmodified
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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