- 商品介绍
- 规格参数
- 包装参数
General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgG.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody reacts with mouse IgG in vitro and in mouse serum and biological fluids. It does not react with human serum proteins.
Immunogen
Purified mouse IgG
Application
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in peptide arrays (1:500) and western blot. The product can also be used for direct ELISA (1:10,000).
Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. Immunostaining was performed using HRP-conjugated sheep anti-mouse IgG (SAM) diluted in 1% BSA-TBS. HRP was visualized using Na nitroprusside and o-clianisidine (Sigma).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Legal Information
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | sheep |
| Quality Segment | 200 |
| conjugate | peroxidase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| technique(s) | direct ELISA: 1:10,000 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 3109.49
-
询价
-
询价
-
询价
-
询价
-
¥ 238.17
-
询价
-
瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
-
¥ 15214.65
-
¥ 4404.38
-
¥ 1476.00
-
¥ 1476.40
-
¥ 5804.96
-
¥ 2288.54
-
NNALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/4 x 1/2 x 1/8，英寸，每箱数量50，50/箱，8000-0080，Thermofisher，赛默飞世尔
询价
-
¥ 70526.15
-
¥ 64659.66
-
¥ 165230.53
-
¥ 145427.01
微信小程序
7X24小时在线咨询