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General description
ATP-binding cassette sub-family C member 2 (UniProt: Q92887; also known as Canalicular multidrug resistance protein, Canalicular multi-specific organic anion transporter 1, Multidrug resistance-associated protein 2, MRP2) is encoded by the ABCC2 (also known as CMOAT, CMOAT1, CMRP, MRP2) gene (Gene ID: 1244) in human. MRP2, a member of the family of human ATP-binding cassette (ABC) transporters, binds and hydrolyzes ATP to enable active transport of various substrates, including many drugs, toxicants, and endogenous compound across cell membranes. It transports a wide variety of conjugated organic anions such as sulfate-, glucuronide- and glutathione (GSH)-conjugates of endo- and xenobiotics substrates. It is reported to mediate hepatobiliary excretion of mono- and bis-glucuronidated bilirubin molecules. Hence, it plays an important role in bilirubin detoxification. MRP2 is expressed in the apical hepatocyte plasma membrane, renal proximal tubules, and small intestine, where it plays a role in the elimination and oral bioavailability of drugs, xenotoxins, and their metabolites. It also confers resistance to several anti-cancer drugs, including cisplatin, doxorubicin, epirubicin, methotrexate, etoposide, and vincristine. It contains two conserved nucleotide-binding domains (aa 671-678 and 1334-1341) that mediate the binding and hydrolyzing ATP via an ATPase, thereby providing energy for substrates export from the cytoplasm to the extracellular space. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hayashi, H., et al. (2005). Biochim. Biophys. Acta. 1738(1-3); 54-62; Cui, Y., et al. (1999). Mol. Pharmacol. 55(5); 929-937; Kamisako, T., et al. (1999). Hepatology. 30(2); 485-490).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal, cytoplasmic domain of human Multidrug resistance-associated protein 2 ( MRP2).
Application
Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRP2 in A549 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRP2 in Human liver tissue lysate.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected MRP2 in one million A549 cells.
Affinity Binding Assay: A representative lot of this antibody bound MRP2 peptide with a KD of 1.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRP2 in A549 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected MRP2 in human liver tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MRP2 in human liver tissue, iPSC-derived liver organoidprogenitor, and mature liver organoid sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1B2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Multidrug resistance-associated protein 2 ( MRP2). It targets an epitope within 14 amino acids from the C-terminal, cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B2, recombinant monoclonal |
| description | 1B2 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 174.21 kDa,observed mol wt ~200 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_000383 |
| UniProt accession no. | Q92887 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ABCC2(1244) |
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