Anti-CD137 antibody produced in goat，0.1MG，C0730-.1MG，Sigma

General description

CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.

Immunogen

mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu²⁴Gln¹⁸⁶

Application

Anti-CD137 antibody produced in goat is suitable for the following applications:

• Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
• ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
• Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
• Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
• Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.

Biochem/physiol Actions

CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Reconstitute at 0.2 mg/mL in sterile PBS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.