Anti-GTF2F2 antibody produced in rabbit，100UL，HPA006912-100UL，Sigma

Immunogen

Transcription initiation factor IIF subunit β recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

GTF2F2 (General transcription factor IIF, polypeptide 2, 30kDa) is a α2β2 heteromeric general transcription initiation factor with RNA polymerase II binding capacity. It is a member of the "winged" helix-turn-helix family of eukaryotic transcription factors. It is majorly involved in the various transcriptional processes including initiation, elongation, and recycling of transcription. Its ATP-dependent DNA helicase motif helps to melt or untwist the DNA strand at the transcriptional initiation point. During transcription, it directly binds to the RNA polymerase II subunit 5 (RPB5) to modulate the initiation and/or elongation of mRNA synthesis.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST71013

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany