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Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.，0.25UNIT，S2563-.25UN，Sigma

销售价： ¥ 5255.04 / 件

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订货号： 0BV0045

品牌型号： Sigma S2563-.25UN

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最小订货量： 1件

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产品介绍 Product Description

General description

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.

Application

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.

Biochem/physiol Actions

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.
Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.

Physical form

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

Other Notes

One unit will hydrolyze 1 μmol of asialo GM₁ per minute at pH 6.0 at 37 °C.

技术参数 Specifications

Quality Segment	200
foreign activity	exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free,protease and sphingomyelinase, essentially free
shipped in	dry ice
storage temp.	−20°C

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