脲酶缓冲试剂,1VIAL,U3383-1VL,Sigma

销售价: ¥ 717.65 / 件
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订货号 0BV2203
品牌型号 Sigma U3383-1VL
货期 询货期
最小订货量 1件
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  • 商品介绍
  • 规格参数
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产品介绍 Product Description

General description

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.


Application

Urease Buffer Reagent has been used:

  • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
  • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
  • to study the metabolic capacity of rat hepatocytes


Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.


技术参数 Specifications
descriptionWhen reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Quality Level200
formpowder
mol wt 545 kDa
storage temp.2-8°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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