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General description
Upon entry into Mitotic (M) phase of cell cycle, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The reversible phosphorylation of proteins regulates transitions between various phases of the cell cycle and the cytoarchitectural changes necessary for cell division. One such group of proteins is the family of MAP kinases that play critical roles in cell cycle control. They mediate intracellular signal transmission in response to external stimuli. p42MAP kinase requires phosphorylation on both threonine 183 and tyrosine 185 for its full activation. This MPM-2 recombinant monoclonal antibody binds to a phospho amino acid-containing epitopes present on several proteins of M-phase eukaryotic cells. It recognizes phosphorylated serine/threonine epitope that is followed by a proline residue. It is reported to recognizes the activated form of p42 MAP kinase. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, o
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
Mitotic HeLa cell cytosolic lysate.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in Human skin tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected phospho-S/T-Pro MPM-2 in Human skin tissue sections.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in Human prostate cancer tissue sections
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-S/T-Pro MPM-2 in HeLa cells treated with colcemid.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone MPM-2 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects phosphorylated serines/threonines when followed by a proline in mitotic proteins.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MPM-2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt Variable kDa,observed mol wt ~50-180 kDa |
| purified by | using protein G |
| species reactivity | human |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | western blot: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable |
| isotype | IgG1 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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