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General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes, and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
~32 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Monomethyl Histone H1 (Lys26).
Application
Detect Histone H1 using this rabbit polyclonal antibody, Anti-Monomethyl Histone H1 (Lys26) Antibody validated for use in western blotting, Dot Blot & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Monomethyl Histone H1 (Lys26) in 500 µg of HeLa acid extract.
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance
Biochem/physiol Actions
This antibody recognizes the N-terminus of Monomethyl Histone H1 (Lys26) methylated at Lys26.
Analysis Note
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | dot blot: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_005311 |
| UniProt accession no. | P16402 |
| target post-translational modification | monomethylation (Lys26) |
| Gene Information | human ... HIST1H1D(3007) |
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