Anti-Integrin α4 Antibody, cytoplasmic on CT,100UL,AB1924,Sigma

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订货号 0BV3125
品牌型号 Sigma AB1924
货期 询货期
最小订货量 1件
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产品介绍 Product Description

Immunogen

Epitope: cytoplasmic domain, C-terminus
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.


Application

Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
ELISA/RIA: 1:1000.

Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

Immunohistochemistry: 1:500

Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins


Biochem/physiol Actions

Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.


Physical form

Liquid rabbit antiserum with 0.05% sodium azide.


Preparation Note

Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.


Other Notes

Replaces: 04-1131


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
conjugateunconjugated
antibody formserum
antibody product typeprimary antibodies
clonepolyclonal
species reactivityhuman, mouse, chicken, hamster, rat, pig
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,radioimmunoassay: suitable,western blot: suitable
NCBI accession no.NM_000885.4
UniProt accession no.P13612
shipped inwet ice
target post-translational modificationunmodified
Gene Informationchicken ... Itga4(424121)
human ... ITGA4(3676)
mouse ... Itga4(16401)
pig ... Itga4(100521477)
rat ... Itga4(311144)
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