Anti-Rictor Antibody, clone 9F1.2,100UG,05-1471,Sigma

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订货号 0BV3283
品牌型号 Sigma 05-1471
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

190 kDa Observed
Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.


Immunogen

Recombinant protein corresponding to human Rictor.


Application

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.


Biochem/physiol Actions

This antibody recognizes Rictor.


Physical form

Format: Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.


Analysis Note

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.
Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.


Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


技术参数 Specifications
biological sourcemouse
Quality Level100
conjugateunconjugated
antibody formpurified immunoglobulin
antibody product typeprimary antibodies
clone9F1.2, monoclonal
species reactivityhuman, mouse, rat
technique(s)immunoprecipitation (IP): suitable,western blot: suitable
isotypeIgG2aκ
NCBI accession no.NP_689969.2
UniProt accession no.Q6R327
shipped inwet ice
target post-translational modificationunmodified
Gene Informationhuman ... RICTOR(253260)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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