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General description
Nuclear lamins are composed of the type V intermediate filament proteins. Often referred to as nucleoskeletal proteins they play a key role in nuclear integrity, positioning of nuclear pores, and overall nuclear size and shape. They also play several key functional roles in the maintenance and propagation of the genome--replication, transcription-- as well as the disassembly and reassembly of the nucleus during cell division. In vitro studies have shown that dimers are the basic building blocks of higher order lamin structures and in low concentrations lamins are distributed throughout the nucleoplasm. In humans, there are two types of lamins: A-type lamins (lamins A and C), found primarily in differentiated cells, and B-type lamins (lamins B1 and B2), found in all nucleated cells. Nuclear lamins are involved in a number of essential nuclear functions, including nuclear envelope assembly and disassembly during cell division, DNA synthesis, transcription, and apoptosis. Nuclear lamins have been found to co-localize with DNA synthesis sites.
~74/65 kDa
Immunogen
Peptide from human lamin A/C corresponding to amino acids 398 to 490.
Application
Anti-Lamin A/C Antibody, clone 14 detects level of Lamin A/C & has been published & validated for use in IC & WB.
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes Lamin A, MW ~74 kDa and Lamin C, MW ~65 kDa.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 50% storage buffer (20 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 1 mg/mL BSA, 0.09% sodium azide) and 50% glycerol. Store at -20°C.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Evaluated by western blot on RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected Lamin A/C in 20 μg of RIPA lysates from A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE481
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 14, monoclonal |
| species reactivity | canine, chicken, human, rat, mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_005572 |
| UniProt accession no. | P02545 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LMNA(4000) |
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