- 商品介绍
- 规格参数
- 包装参数
General description
Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimEL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.
Application
Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
Biochem/physiol Actions
Rat BimEL when phosphorylated at serine 65. Human BimEL when phosphorylated at serine 69. Mouse BimEL (100% conserved) has not been tested, but is expected to react.
Physical form
Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
Preparation Note
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Analysis Note
Control
Hek293T co-expressing rat BimEL and active MEKK1 (MEKK1δ).
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a BimEL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, human |
| species reactivity (predicted by homology) | mouse (100% homologous) |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_006538.3,NM_138621.3,NM_138622.2,NM_138623.2,NM_138624.2,NM_138626.2,NM_138627.2,NM_207002.2,NM_207003.1 |
| UniProt accession no. | O43521 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pSer69) |
| Gene Information | human ... BCL2L11(10018) |
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