Anti-Histone H3.3 Antibody, K27M mutant，100UG，ABE419，Sigma

General description

Histone H3.3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3.3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
~17 kDa observed

Immunogen

Epitope: Histsone H3 sequence surrounding K27M mutation
KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3.3 with K27M mutation.

Application

• Peptide Inhibition Assay (PIA): Target band detection inlysate from HEK-293 cells transfected with Histone H3.3 K27M mutant wasprevented by pre-blocking of a representative lot with the immunogen HistoneH3.3 K27M mutant peptide, but not the corresponding unmodified Histone H3.3 peptide.
• Immunohistochemistry (IHC): A representative lot of this antibodydetected Histone 3.3 K27M in pediatric glioblastoma tissue sections. (Venneti,S., et al. (2014). Acta Neuropathol 128(5); 743-753).

Biochem/physiol Actions

This antibody recognizes Histone H3.3 with K27M mutation.

Physical form

Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Evaluated by Western Blotting in HEK-293 cellstransfected with Histone H3.3 K27M.

Western Blotting Analysis (WB): A 1:2,000 dilution of a representative lot of thisantibody detected Histone H3.3 K27M in HEK-293 cells transfected with HistoneH3.3 K27M mutant.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.