- 商品介绍
- 规格参数
- 包装参数
General description
重组人拓扑异构酶I融合到His•Tag
Biochem/physiol Actions
0.1-1单位(ng)足以在20 µl反应中松弛0.5 µg pBR322超螺旋DNA。
Packaging
特定浓度批号请参考小瓶的标签。
Physical form
在100 mM KCl、20 mM Tris-HCl、1 mM DTT、200 µM EDTA、20%甘油、pH 7.9和1瓶稀释缓冲液(100 mM KCl、20 mM Tris-HCl、1 mM DTT、0.2 mM EDTA、20%甘油、pH 8.0)中添加5000 U酶。
Preparation Note
初次融化后,分装并冷冻(-70°C)。对于稀释蛋白质的贮藏,在贮藏缓冲液中用500µg/ml分子生物学级BSA稀释、分装并冷冻(-70°C)。
Other Notes
Pommier, Y., et al. 1998.Biochim.Biophys.Acta1400, 83.
Pourquier, P., et al. 1997.J. Biol. Chem.272, 26441.
Rossi, F., et al. 1996.Nature381, 80.
Sekiguchi, J., et al. 1996.Proc.Natl.Acad.Sci. USA93, 785.
Giovanella, B.C., et al. 1989.Science246, 1046.
Wang, J,C.1985.Annu.Rev. Biochem.54, 665.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
| recombinant | expressed in insect cells (S. frugiperda) |
| Quality Level | 100 |
| assay | ≥95% (SDS-PAGE) |
| form | liquid |
| specific activity | 1 units/ng protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| shipped in | wet ice |
| storage temp. | −70°C |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |




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