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Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Trial Size，10UL，05-745R-S，Sigma

销售价： ¥ 1193.47 / 件

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订货号： 0BV3409

品牌型号： Sigma 05-745R-S

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最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

17 kDa
Recombinant version of Cat. # 05-745, clone MC315

Immunogen

BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.

Application

ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 3 µL of Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 05-745R-S), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 05-745R and 07-473 datasets showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Recombinant, Trial Size (rabbit monoclonal antibody) proven in WB, ChIP, ChIP-seq, ELISA, PIA, Mplex, DB to detect trimethyl-Histone H3 (Lys4) also known as H3K4me3.

Biochem/physiol Actions

Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See certificate of analysis for further details.

Physical form

Format: Purified
Protein A purified, recombinant antibody in 60 mM Tris, 90 mM NaCl, 0.03% NaN3, 60 mM Glycine, pH 7.5, 40% Glycerol.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Routinely evaluated by immunoblot on HeLa nuclear extract

Other Notes

Replaces: 05-745

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	rabbit
Quality Level	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	15-10C-E4, monoclonal
species reactivity	human
manufacturer/tradename	Upstate^®
technique(s)	ChIP: suitable (ChIP-seq),ELISA: suitable,dot blot: suitable,inhibition assay: suitable (peptide),multiplexing: suitable,western blot: suitable
isotype	IgG
NCBI accession no.	NM_003493
UniProt accession no.	Q16695
shipped in	wet ice
target post-translational modification	trimethylation (Lys4)
Gene Information	human ... H3F3B(3021)

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