- 商品介绍
- 规格参数
- 包装参数
Immunogen
Carboxyl fragment of APP 643-695 / Jonas.
Epitope: APP 643-695 C-terminal fragment
Application
Research Sub Category
Neurodegenerative Diseases
This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.
Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.
Western blot: 10 μg/mL
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB343
IMMUNOHISTOCHEMISTRY
1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).
2) Wash sections 3 times in distilled water.
3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.
4) Wash sections for 5 min in TBS.
5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.
6) Wash sections 3 times in TBS for 5 min..
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in TBS.
9) Embed sections and examine.
Research Category
Neuroscience
Biochem/physiol Actions
Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html
Physical form
Liquid.
Unpurified
Preparation Note
Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Brain
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| clone | 2.F2.19B4, monoclonal |
| species reactivity | rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_000484.2,NM_201413.1,NM_201414.1 |
| UniProt accession no. | P05067 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... APP(351) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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