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General description
DBF4, also known as Protein DBF4 homolog A, Activator of S phase kinase, also known as Chiffon homolog A or DBF4-type zinc finger containing protein 1, and encoded by the gene DBF4/ASK/DBF4A/ZDBF1, is the regulatory subunit for the cell cycle kinase CDC7. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at ′Ser-40′ and ′Ser-53′ and then is involved in regulating the initiation of DNA replication during cell cycle. . DBF4 Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 proteins. DBF4 is localized to the nucleus. DBF4 is expressed in expressed in many cells particularly high in testis, thymus and most cancer cells.
~77 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Near C-terminus
GST-tagged recombinant protein corresponding to human DBF4 near the C-terminus.
Application
Anti-DBF4 antibody, clone 7H8.1 detects level of DBF4 & has been published & validated for use in DBF4.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DBF4 in 200 µg of human thymus tissue lysate.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DBF4 in 200 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 7H8.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_006707 |
| UniProt accession no. | Q9UBU7 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DBF4(10926) |
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