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General description
BMPR-1A (bone morphogenic protein receptor type 1A) signaling is known to be important for inner root sheath differentiation, hair follicle cycling and tooth development. Mutations in BMPR-1A are a common cause of juvenile polyposis syndrome (JPS). BMPR-1A is expressed in a high quantity during the formation of the primary and secondary palate and the loss of BMPR-1A could be a cause for the cleft palate birth defect.
~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human BMPR1A expressed in E. Coli
Application
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected BMPR-1A in colon cancer and kidney tissues.
Flow Cytometry Analysis: A 1;200-400 dilution from a representative lot detected BMPR-1A in HeLa cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Signaling
Research Sub Category
Developmental Signaling
This Anti-BMPR-1A, clone 4B7B2 Antibody is validated for use in western blotting, IHC, flow cytometry & ELISA for the detection of BMPR-1A.
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in PC-3, K562 and mouse liver lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected BMPR-1A in PC-3, K562 and mouse liver lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 4B7B2, monoclonal |
| species reactivity | mouse, human |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P36894 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BMPR1A(657) |
| 长度(mm) | |
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| 重量(kg) |
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NALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/8 x 1/4 x 1/16，英寸，每箱数量50，50/箱，8000-0020，Thermofisher，赛默飞世尔
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