Introductory pSTBlue-1 Perfectly Blunt® Cloning Kit - Novagen，10REACTIONS，70184-3CN，Sigma

销售价： ¥ 2507.96 / 件

在线询价

优惠：请询价

订货号： 0BV3762

品牌型号： Sigma 70184-3CN

货期：询货期

最小订货量： 1件

+ -

加入购物车

联系我们

400-920-3909

微信扫一扫，手机采购

精选商品

SLC4A3 100 UL，PA551351，赛默飞世尔

销售价：询价

加入购物车

SC-TOPO TA/ DUAL/ TOP10 25 RXN，K460001SC，Invitrogen

销售价：询价

加入购物车

ANTI-PADI2/PAD2 EA，PA519474，赛默飞世尔

销售价：询价

加入购物车

AVIDIN, NEUTRAVIDIN, TEXAS RED 1 MG，A2665，Invitrogen

销售价：询价

加入购物车

ANTI-MO CD357 DTA-1 PE 100UG，12-5874-82，Affymetrix

销售价：询价

加入购物车

ANTI-RPL11 EA，PA534604，赛默飞世尔

销售价：询价

加入购物车

商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

The Perfectly Blunt^® Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed (Novy 1996, Clark 1998, Brownstein 1996, Magnuson, 1996, Hu 1993).

With the Perfectly Blunt cloning protocol, you can go from PCR product to plating transformants in less than one hour with minimal hands-on time. The finished PCR product is converted to a blunt, phosphorylated form in a 15-minute reaction using premixed reagents. Following a 5-minute heat inactivation step, the treated insert is combined with the ready-to-use vector and ligated in an optimized 15-minute reaction. An exclusive 8-minute transformation procedure using highly efficient NovaBlue Singles Competent Cells (Cat. No. 70181) generates recombinant colonies that are easily visualized by blue/white screening.

Note that the Perfectly Blunt method is not limited to cloning PCR products; these kits are also suitable for cloning restriction fragments, cDNA, or sheared DNA with the same protocols.

Seven different vectors are available in Perfectly Blunt^® Cloning Kits. Vector choices include those designed for general cloning, sequencing, optimal in vitro transcription/translation, and optimal protein expression in E. coli. Each vector is available in a kit containing sufficient reagents for 10, 20, or 40 reactions.

“Vector only” kits are also available in 20- and 40-reaction sizes without ligase and competent cells. For higher efficiency competent cells, also consider pSTBlue-1 Perfectly Blunt^® Giga Cloning Kit (Cat. No. 71229).

The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.

Other Notes

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.