CpGenome 5-mC and 5-hmC DNA Standard Set,3X2UG,S8005,Sigma

销售价: ¥ 5117.93 / 件
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订货号 0BV3773
品牌型号 Sigma S8005
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

CpGenome 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.

Materials Provided:

Three vials, each containing 2 µg (40 µL) of CpGenome Cytosine DNA Standard, CpGenome 5-mC DNA Standard, or CpGenome 5-hmC DNA Standard, at a concentration of 50 ng/μL.

Validation:

The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.


Physical form

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0


Preparation Note

Recommended Storage: Stable for 6 months at -20°C from date of receipt.


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
species reactivityhuman
Quality Segment100,300
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