- 商品介绍
- 规格参数
- 包装参数
General description
Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa
This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.
Immunogen
The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.
Application
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select
detection with HRP-DAB.
Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Biochem/physiol Actions
Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.
Physical form
Format: Purified
Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Jurkat Cell Lysate
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4091
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, bovine, human |
| technique(s) | immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_002721.3 |
| UniProt accession no. | O00743 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP6C(5537) |
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| 重量(kg) |
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