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General description
30.50 kDa calculated.
T-cell surface glycoprotein YE1/48 (UniProt: P20937; also known as Lymphocyte antigen 49a, Ly-49a, T lymphocyte antigen A1) is encoded by the Klra1 (also known as Ly-49, Ly-49a, Ly49, Ly49A) gene in murine species. LY49A is a single-pass type II membrane protein that serves as an inhibitory receptor on natural killer (NK) cells for H-2d(Dd) MHC class I molecules on target cells. It inhibits the activity of NK cells thereby preventing cell lysis. Ly49A-mediated NK cell inhibition is shown to be dependent on a lateral binding site, which is located beneath the peptide-binding platform of Dd. Ly49A is reported to contribute to target cell adhesion and accumulate at synapses with Dd-expressing target cells when NK cells themselves lack Dd. In cases where NK cells display Dd expression Ly49A does not contribute to adhesion. Higher expression of LY49A is observed in T-lymphoma cell lines and normal lymphocytes exhibit low expression. LY49A has a cytoplasmic domain (aa 1-44), a transmembrane domain (aa 45-66), and an extracellular domain (aa 67-262). (Ref.: Back, J., et al. (2007). Proc. Natl. Acad. Sci. USA 104(10); 3978 3983).
Immunogen
EL-4 T lymphoma cells.
Application
Immunoprecipitation Analysis: A representative lot detected Ly49A in Immunoprecipitation applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86).
Immunocytochemistry Analysis: A representative lot detected Ly49A in Immunocytochemistry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83).
Flow Cytometry Analysis: A representative lot detected Ly49A in Flow Cytometry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83; Silver, E.T., et. al. (2000). J Immunol. 165(4):1771-81).
This mouse monoclonal Anti-Ly49A, clone A1, Cat. No. MABF2090 is tested for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation, for the detection of Lymphocyte antigen 49a.
Biochem/physiol Actions
Clone A1 specifically detects Lymphocyte antigen 49a (LY49A) in murine species.
Physical form
Format: Purified
Analysis Note
Evaluated by Flow Cytometry in EL-4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Ly49A in 1X10E6 EL-4 T lymphoma cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | A1, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2aκ |
| UniProt accession no. | P20937 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Klra1(16627) |
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