- 商品介绍
- 规格参数
- 包装参数
General description
35 kDa was observed; however, the calculated molecular weight is 31.849 kDa
The survival of motor neurons (SMN) protein is essential for the biogenesis of small nuclear RNA (snRNA)-ribonucleoproteins (snRNPs), the major components of the pre-mRNA splicing machinery. Though it is ubiquitously expressed, SMN deficiency causes the motor neuron degenerative disease spinal muscular atrophy (SMA). SMN deficiency, similar to that which occurs in severe SMA, has unexpected cell type-specific effects on the repertoire of snRNAs and mRNAs. It alters the stoichiometry of snRNAs and causes widespread pre-mRNA splicing defects in numerous transcripts of diverse genes, preferentially those containing a large number of introns, in SMN-deficient mouse tissues. These findings reveal a key role for the SMN complex in RNA metabolism and in splicing regulation and indicate that SMA is a general splicing disease that is not restricted to motor neurons.
Immunogen
Epitope: Unknown
His-tagged recombinant protein corresponding to human SMN.
Application
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SMN in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Use Anti-SMN Antibody, clone 62E7 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect SMN also known as Spinal muscular atrophy (Werdnig-Hoffmann disease Kugelberg-Welander disease).
Biochem/physiol Actions
Recognizes SMN.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SMN on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 62E7, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse, Xenopus |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000335.1 |
| UniProt accession no. | Q16637 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SMA4(11039) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 2528.88
-
¥ 2019.57
-
¥ 4039.86
-
询价
-
询价
-
询价
-
¥ 3442.25
-
¥ 9737.84
-
询价
-
询价
-
询价
-
¥ 20380.83
-
¥ 2617.87
-
¥ 2617.87
-
¥ 2307.00
-
¥ 1196.00
-
¥ 1586.69
-
¥ 70526.15
-
¥ 165230.53
-
询价
微信小程序
7X24小时在线咨询