Anti-Dopamine D₃ Receptor Rabbit pAb，100UL，324402-100ULCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D₃ dopamine receptor protein.
Recognizes the dopamine D₃ receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor

Application

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:500)

Immunohistochemistry (1:5000)

Preparation Note

Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Other Notes

This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	rabbit
Quality Segment	100
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
form	lyophilized
does not contain	preservative
species reactivity	rat, human
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze
dilution	(ELISA Flow Cytometry (1:100, Immunoblotting (1:500) Immunohistochemistry (1:5000))
isotype	IgG
shipped in	ambient
storage temp.	2-8°C
target post-translational modification	unmodified
Gene Information	human ... DRD3(1814) rat ... Drd3(29238)

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