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General description
Disks large homolog 4 (UniProt: P31016; also known as Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90) is encoded by the Dlg4 (also known as Dlgh4, Psd95) gene (Gene ID: 29495) in rat species. PSD95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. The assembly of the GluR6-PSD95-CaMKII complex is reported to mediate injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.
~95 kDa observed; 80.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat PSD95 protein.
Application
Anti-PSD95, clone 3H4.3, Cat. No. MABN1194, is a highly specific mouse monoclonal antibody that targets PSD95 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PSD95 in human cerebellum, rat cerebellum, and rat brain stem tissues.
Research Category
Neuroscience
Biochem/physiol Actions
Clone 3H4.3 detects PSD95 in human and mouse brain.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD-95 in 10 µg of mouse brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3H4.3, monoclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_062567 |
| UniProt accession no. | P31016 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... DLG4(1742) rat ... Dlg4(29495) |
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