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General description
N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 - 765 containing the V561 mutation. The coordinates for recombinant human FGFR1 were described in Homann et al. (2001). Blencke et al. (2004) suggested mutations to a conserved threonine residue at the ATP binding site would result in inhibitor resistance. The amino acid valine 561 was mutated to a methionine in FGFR1 which corresponded to previously reported mutations found in Abl (T315) and EGFR (T766) which had been shown to confer resistance to selective inhibitors. Assay data for FGFR1 V561M had shown that this mutation had conferred resistance to PP58 (pyrido [2,3-d] pyrimidine tyrosine kinase inhibitor) compared to that of the wild type.
Product Source: Recombinant human FGFR1 expressed by baculovirus in Sf21 cells.
Application
Research Category
Metabolism
Research Sub Category
Obesity
Metabolic Disorders
Biochem/physiol Actions
Protein Target: FGFR1
Target Sub-Family: TK
Physical form
Glutathione-agarose
Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Preparation Note
Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Analysis Note
Routinely evaluated by phosphorylation of 500μM GGEEEEYFELVKKKK
Other Notes
Concentration: Please see lot specific Certificarte of Analysis.
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Please see lot specific Certificarte of Analysis.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| mol wt | Mw 62.3 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable |
| NCBI accession no. | NM_015850.3,NM_023105.2,NM_023106.2,NM_023107.2,NM_023108.2,NM_023110.2,NM_023111.2 |
| UniProt accession no. | P11362 |
| Gene Information | human ... FGFR1(2260) |
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