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General description
Partitioning defective 3 homolog (UniProt Q8TEW0; also known as ASIP, Atypical PKC isotype-specific-interacting protein, Bazooka, CTCL tumor antigen se2-5, Par-3 family cell polarity regulator alpha, Par-3 partitioning defective 3 homolog, PAR3-alpha, Protein phosphatase 1 regulatory subunit 118) is encoded by the PARD3 (also known as Baz, PAR3, PAR3A, PARD3A, PPP1R118) gene (Gene ID 56288) in human. Par3 is a PDZ (PSD95/Dlg/ZO-1) domain-containing membrane scaffold adapter protein that plays an important role in epithelial cell tight junction (TJ) formation and apico-basal polarity maintenance. Together with PAR6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, Par3 forms a complex that regulates cytoskeleton re-organization, post-golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PAR6 interacts directly with aPKC isoforms iota/lambda and zeta to form the complex core. Recruitment of GTP-bound CDC42 to the PAR6-aPKC core induces aPKC phosphorylation/activation by PAR6, and subsequent PAR3 binding. Another quaternary apical junctional complex (AJC), Willin–Par6-aPKC–Par3, has also been reported. In this case, the complex-associated aPKC functions to phosphorylate ROCK, resulting in ROCK dissociation from the AJC and thereby preventing excessive ROCK-dependent apical constriction.
~180, 150, 100 kDa observed
Immunogen
GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Partitioning-defective 3 in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-330) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry applications.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Partitioning-defective 3 Antibody, Alexa Fluor™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3.
Biochem/physiol Actions
Expected to react with all 11 spliced isofoms (UniProt Q8TEW0-1 to Q8TEW0-11).
Physical form
Protein A purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HUVECs.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Partitioning-defective 3 in HUVECs.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | monkey, canine, human, rat, frog, mouse |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_062565 |
| UniProt accession no. | Q8TEW0 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PARD3(56288) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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