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General description
Tumor necrosis factor receptor superfamily member 10A (UniProt: O00220; also known as Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD261) is encoded by the TNFRSF10A (also known as APO2, DR4, TRAILR1) gene (Gene ID: 8797) in human. CD261/DR4 is a single-pass type I membrane protein, which is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-239), a transmembrane domain (aa 240-262), and a cytoplasmic domain (aa 263-468). It is widely expressed with high levels observed in spleen, peripheral blood leukocytes, small intestine, and thymus. TRAIL receptors (DR4 and DR5) induce apoptosis via caspase activation. The adapter molecule FADD is reported to recruit caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation that initiates the subsequent cascade of caspases mediating apoptosis. O-GlcNAcylation of DR4 is reported to be crucial for TRAIL death signaling. Both TRAIL-sensitive and TRAIL-resistant breast cancer cell lines can be efficiently killed by overexpression of the CD261/DR4. However, the extent of cell death depends on the strength of the promoter driving DR4 expression. (Ref.: Lee, H., et al. (2019). Cancer Res. 79; 2839-2852; Kazhdan, I., and Marciniak, RA (2004). Cancer Gene Ther. 11(10); 691-698).
Immunogen
Recombinant fragment corresponding to the extracellular domain of human DR4/CD261 Fc fusion protein.
Application
Quality Control Applications
Evaluated by Flow Cytometry in BHK cells transfected with Human DR4.
Flow Cytometry Analysis: 1.0 µg of this antibody detected DR4/CD261 in BHK cells transfected with Human DR4.
Tested applications
Flow Cytometry: A representative lot detected DR4/CD261 in Flow Cytometry applications (Uno, K., et al. (2003). Blood. 101(9); 3658-67; Lee, H., et al. (2019). Cancer Res. 79(11); 2839-2852).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone DJR1 is a mouse monoclonal antibody that detects DR4/CD261. It targets an epitope within the extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | DJR1, monoclonal |
| mol wt | calculated mol wt 50.09 kDa |
| purified by | using protein G |
| species reactivity | human |
| packaging | antibody small pack of 100 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_003835 |
| UniProt accession no. | O00220 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TNFRSF10A(8797) |
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询价
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