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General description
Neuron-specific protein family member 1 (NSG1), is also known as Brain neuron cytoplasmic protein 1. It is responsible for dopamine receptor binding in the dopamine signaling pathway.
~20/17 kDa observed. An uncharacterized band may appear at ~12 kDa in some lysates. The calculated molecular weight is 21 kDa, however NSG1 has been shown as a ~20/17 kDa band in western blots (Catsicas, S., et al. (2004). Lausanne, EPFL. 1-162).
Immunogen
Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human NSG1.
Application
Detect the NSG1 protein using this Anti-NSG1 validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NSG1 in rat cerebellum, human pons/midbrain, and rat hippocampus tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Biochem/physiol Actions
This antibody recognizes the cytoplasmic domain of NSG1.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human cerebellum tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected NSG1 in 10 µg of human cerebellum tissue lysate.
Control
Human cerebellum tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_055207 |
| UniProt accession no. | P42857 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NSG1(27065) |
| 长度(mm) | |
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