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General description
Microtubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in rat. MAP-2 belong to the family of thermostable proteins associated with microtubules. It is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. It also has a stiffening effect on microtubules. MAP-2 is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly.
~270 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Three KLH-conjugated linear peptides corresponding to the N-terminal and central regions of rat MAP-2 .
Application
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in E18 rat hippocampus neurons.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues.
Anti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Biochem/physiol Actions
Other homologies: Mouse (94% sequence homology). Human (72% sequence homology).
This antibody recognizes MAP-2.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain microsomal tissue lysate
Evaluated by Western Blotting in rat brain microsomal preparation.
Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_037198 |
| UniProt accession no. | P15146 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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¥ 2290.21
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¥ 8520.40
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¥ 8135.09
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¥ 9737.84
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¥ 3812.28
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¥ 15214.65
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