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General description
Septin-9 (also known as eighth septin, Eseptin, and MLL septin-like fusion) is encoded by the Sept9 gene in rat and mouse (SEPT9 in human). Septins are GTP-binding proteins capable of forming higher-order oligomers and filamentous polymers that contact with the cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. The expression of SEPT2, SEPT6, SEPT7 and SEPT9 in heart tissue is reported to correlate well with cardiomyocyte cytokinesis activity during heart development, is highly expressed in embryonic heart, downregulated around birth, and nondetectable in the adult heart. (PMID 16739252 & 24837489). Clone 1A6C2 was generated using an immunogen sequence present only in rat spetin-9 isoform 1 (SLP-a; Q9QZR6), but missing in rat isoform 2-4 (SLP-b, E-septin long & short forms). The immunogen sequence is 100% conserved in murine species (A2A6U3) and differs by one amino acid when compared to the corresponding human sequence in isoform 2, and missing in human isoforms 1,3,4 (Q9UHD8).
~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of rat Septin-9.
Application
Anti-Septin-9 Antibody, clone 1A6C2 is an antibody against Septin-9 for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Septin-9 in HeLa cells (Dr. Taro Tachibana, Cell Engineering Corporation).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Septin-9 in HeLa cell lysate (Dr. Taro Tachibana, Cell Engineering Corporation).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Septin-9 in 10 µg of NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1A6C2, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001106969 |
| UniProt accession no. | Q9QZR6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SEPTIN9(10801) |
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