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Caspase-10, Human, Recombinant, E. coli，3000U，218785-3000UCN，Sigma

销售价： ¥ 6180.41 / 件

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订货号： 0BV4475

品牌型号： Sigma 218785-3000UCN

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最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

recombinant	expressed in E. coli
Quality Segment	100
assay	≥95% (SDS-PAGE)
form	liquid
specific activity	≥150,000 units/mg protein,≥50,000 units/mL
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
shipped in	wet ice
storage temp.	−70°C

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