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General description
Podocalyxin-like protein 1 is a sialomucin that is involved in the regulation of both cell adhesion and cell morphology through its interactions with several intracellular and at least one extracellular ligand. It is known to play an essential role in the formation and maintenance of podocyte foot processes, in cancer development and aggressiveness and as a marker of undifferentiatted embryonic stem cells. MABD151, a monoclonal antibody generated against human iPS cells, is a novel marker antibody recognizing a type of keratan sulfate lacking oversulfated structure on hiPS cells. MABD151 is a useful molecular probe for disclosing the roles of glycans on the surface of hiPS cells in the maintenance of self-renewal and pluripotency and also as a potent tool for the evaluation and standardization of hiPS cells with different tissue origins and different histories in regenerative medicine (Kawabe K et.al, (2013) Glycobiology. v23, 322–336.
above 250kDa
Immunogen
Whole Human iPS cells (Tic cell line).
Application
Evaluated by Western Blot in human brain lysate.
Western Blot Analysis: 1µg/mL of this antibody detected podocalyxin associated keratin sulphate in 10 µg of human brain lysate.
Evaluated by Immunocytochemistry in human ES cells.
Immunocytochemical Analysis: A 1:250 dilution of this antibody detected odocalyxin associated keratin sulphate in human ES cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-podocalyxin associated keratan sulfate antibody, clone R-10G is validated for use in ICC & western blotting for the detection of podocalyxin associated keratan sulfate.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blot in human ES cell lysate.
Western Blot Analysis: 1µg/mL of this antibody detected podocalyxin associated keratin sulphate in 10 µg of human ES cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | R-10G, monoclonal |
| species reactivity | human |
| should not react with | mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1, kappa |
| NCBI accession no. | NP_001018121 |
| UniProt accession no. | O00592 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PODXL(5420) |
| 长度(mm) | |
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