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General description
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
Immunogen
BSA-conjugated recombinant Hydroxymethylcytosine DNA.
Application
Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.
Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Analysis: A representative lot was used by an independent laboratory to immunoprecipitate 5-Hydroxymethylcytosine DNA usingh chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Biochem/physiol Actions
Expected to detect 5-Hydroxymethylcytosine in all species
This antibody detects 5-Hydroxymethylcytosine, but not 5-Methylcytosine or unmethylated cytosine.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-Hydroxymethylcytosine DNA.
Dot Blot Analysis: A 1:5000 dilution of this antibody detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | HMC-MA01, monoclonal |
| species reactivity (predicted by homology) | all |
| technique(s) | dot blot: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG1κ |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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