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General description
Calcitonin gene-related peptide 1 (UniProt: Q99JA0; also known as CGRP1, Alpha-type CGRP, Calcitonin gene-related peptide I (CGRP-I)) is encoded by the Calca (also known as Calc) gene (Gene ID: 12310) in murine species. Calcitonin gene-related peptide 1 is a neuropeptide derived by tissue specific alternative splicing of the CALCA precursor, which also yields calcitonin and katacalcin, followed by proteolytic maturation of the pre-prohormone in the secretory pathway of peptidergic neurons and endocrine cells. It is a member of the calcitonin peptide family and adopts an N-terminal disulfide constrained loop and C-terminal amphipathic α helix typical of class B GPCR ligands, which enables high affinity binding to the heterodimeric CGRP receptor composed of calcitonin receptor like receptor (CLR, a class B GPCR) and receptor activity–modifying protein 1 (RAMP1). With lower affinity it can also signal via CTR:RAMP1 “AMY1 like” complexes, providing receptor redundancy within the calcitonin/adrenomedullin/amylin family. Calcitonin gene-related peptide 1 is widely expressed in small diameter primary sensory neurons of dorsal root and trigeminal ganglia, in central projections within brainstem nuclei, in subsets of enteric, autonomic and other peripheral neurons and in perivascular sensory fibers innervating meningeal, cerebral, coronary and systemic resistance arteries. Subcellularly, Calcitonin gene-related peptide 1 is stored in large dense core vesicles in peripheral and central nerve terminals and is released in a Ca²⁺ dependent manner upon nociceptor activation, where it acts primarily as a secreted paracrine hormone/neurotransmitter rather than as an intracellular protein. Functionally, Calcitonin gene-related peptide 1 is one of the most potent endogenous microvascular vasodilators known. Engagement of CLR:RAMP1 on vascular smooth muscle and endothelial cells activates Gs–adenylyl cyclase signaling, elevates cAMP, mobilizes intracellular Ca²⁺, and triggers nitric oxide production, resulting in relaxation of cerebral, meningeal and systemic arteries and elevation of platelet cAMP. Beyond vasodilation, Calcitonin gene-related peptide 1 modulates nociceptive transmission and central sensitization in the trigeminovascular system, promotes neurogenic inflammation (mast cell degranulation, plasma extravasation), sensitizes NMDA receptors, and activates pro survival and growth factor pathways, suggesting neuroprotective roles in ischemia–reperfusion injury. Calcitonin gene-related peptide 1 also plays a central, causative role in migraine and other trigeminal autonomic cephalalgias.
Immunogen
KLH-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region of mouse Calcitonin Gene Related Peptide.
Application
Anti-Calcitonin Gene Related Peptide, Cat. No. AB15360, is a rabbit polyclonal antibody that detects Calcitonin gene-related peptide 1. It targets an epitope within 31 amino acids from the C-terminal region.
Evaluated by Immunohistochemistry in rat spinal cord tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:5,000 dilution from a representative lot detected Calcitonin Gene Related Peptide in rat spinal cord tissue sections.
Tested applications
Immunofluorescence Analysis: A 1:4,000 dilution from a representative lot detected Calcitonin Gene Related Peptide in rat spinal cord tissue sections.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Calcitonin Gene Related Peptide in human brain tissue lysate.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.
Research Sub Category
CNS Control of Metabolism
Hormones & Receptors
Biochem/physiol Actions
Target molecular weight ~ 15 kDa observed. 14.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Recognizes Calcitonin Gene Related Peptide (CGRP).
Physical form
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Rabbit serum. Liquid.
Preparation Note
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Replaces: AB1971
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_001033952.1,NM_001033953.1,NM_001741.2 |
| UniProt accession no. | P01258,P10092 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CALCA(796) |
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