Anti-Collagen Types I, II & III Antibody, clone MMCHABC,100UG,MAB1334,Sigma

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订货号 0BV4899
品牌型号 Sigma MAB1334
货期 询货期
最小订货量 1件
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产品介绍 Product Description

Immunogen

Human collagen type I


Application

Anti-Collagen Types I, II & III Antibody, clone MMCHABC detects level of Collagen Types I & has been published & validated for use in ELISA, RIA, IH.
ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.

Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.

Not suitable for use on paraffin embedded sections or immunoblots.

Optimal working dilution must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins


Biochem/physiol Actions

Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.


Physical form

Format: Purified
Purified from murine ascites by PEG precipitation and Sepharose CL-4B chromatography. Lyophilized Powder.


Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcemouse
Quality Segment100
antibody formpurified antibody
antibody product typeprimary antibodies
cloneMMCHABC, monoclonal
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable,immunohistochemistry: suitable,radioimmunoassay: suitable
isotypeIgM
suitabilitynot suitable for Western blot
NCBI accession no.NM_000088.3
UniProt accession no.P02452,P02458,P02461
shipped indry ice
target post-translational modificationunmodified
Gene Informationhuman ... COL1A1(1277)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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