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General description
Telomeric repeat-binding factor 2 (UniProt: Q15554; also known as TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein, TERF2) is encoded by the TERF2 (also known as TRBF2, TRF2) gene (Gene ID: 7014) in human. TERF2 is homodimeric protein that is ubiquitous in its expression. Higher expression levels are reported in spleen, thymus, prostate, uterus, testis, peripheral blood leukocytes, colon, and small intestine. Two soforms of TERF2 are reported that are produced by alternative splicing.TERF2 binds to telomeric double-stranded 5′-TTAGGG-3′ repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. It is also required to recruit a number of factors and enzymes required for telomere protection, including the sheltering complex, TERF2IP/RAP1 and DCLRE1B/Apollo. TEFR2 colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. It is phosphorylated upon DNA damage and the phosphorylated TERF2 is not bound to telomeric DNA, and rapidly localizes to damage sites. Inhibition of TRF2 causes apoptosis in several mammalian cell types and its over-expression is observed in a variety of human cancers.
~60 kDa observed; 59.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Tagged full length recombinant human Telomeric repeat-binding factor 2.
Application
Anti-TRF2, clone T2JKM3, Cat. No. MABE1135, is a highly specific mouse monoclonal antibody that targets Telomeric repeat-binding factor 2 (TRF2) and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected TRF2 in IMR90 primary human fibroblasts.
Western Blotting Analysis: A 1:200 dilution from a representative lot detected TRF2 in HT1080 cell lysate.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
Clone T2JKM3 detects Telomeric repeat-binding factor 2 in human cells.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HT1080 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TRF2 in HT1080 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | T2JKM3, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_005643 |
| UniProt accession no. | Q15554 |
| target post-translational modification | unmodified |
| Gene Information | human ... TERF2(7014) |
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¥ 4039.86
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¥ 4173.75
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¥ 5725.45
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¥ 10277.12
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¥ 4011.97
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