PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb,50UL,DR1025-50ULCN,Sigma

销售价: ¥ 3054.53 / 件
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订货号 0BV4924
品牌型号 Sigma DR1025-50ULCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).


Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1


Application


Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)


Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.


Preparation Note

Do not aliquot.


Analysis Note

Positive Control
Lysates from COS cells treated with UV


Other Notes

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formaffinity isolated antibody
antibody product typeprimary antibodies
clonepolyclonal
formliquid
does not contain preservative
species reactivitymonkey, rat, mouse, human
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze
dilution (Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50))
isotypeIgG
shipped inwet ice
storage temp.−20°C
target post-translational modificationphosphorylation (pSer317/pSer317)
Gene Informationhuman ... CHEK1(1111)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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