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General description
Laminins are secreted glycoproteins, and are an important structural component of the extracellular matrix. The family of laminins is comprised of multiple isoforms, each formed from the oligomerization of various alpha, beta, and gamma chains. Laminin isoforms are expressed in a tissue-specific pattern, and contain multiple domains, which may include laminin EGF-like domains; laminin G-domains; laminin IV type A domains; and a laminin N-terminal domain. Laminins are capable of self-assembly to form a structural scaffold; they also bind to cell-surface molecules such as dystroglycan and mediate attachment of these molecules to the extracellular matrix. Laminins may also participate in intracellular signaling processes by binding to various integrin receptors. They are involved in cell adhesion, migration, differentiation, neurite outgrowth, and metastasis.
~170 kDa observed
Immunogen
Laminin isolated from mouse EHS tumor
Application
Anti-Laminin α1/β1 Antibody, clone AL-4 is an antibody against Laminin α1/β1 for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Biochem/physiol Actions
As originally characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin α1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele, et al. (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kDa β1/γ1 band and the 400 kDa α1 band of mouse EHS laminin. However the affinity for the 400 kDa band was very low. AL-4 also demonstrated strong binding the Laminin β1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin β1 chain, however the clone also recognizes the laminin α1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins α1 and β1, with the β1 reactivity being more prominent.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Laminin purified protein
Evaluated by Western Blot in Laminin purified protein.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Laminin α1/β1 in 10 µg of Laminin purified protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB1905
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | AL-4, monoclonal |
| species reactivity | mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_032506 |
| UniProt accession no. | P19137 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Lama1(16772) |
| 长度(mm) | |
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| 重量(kg) |
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