Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda,50UG,382184-50UGCN,Sigma

销售价: ¥ 7290.01 / 件
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订货号 0BV5018
品牌型号 Sigma 382184-50UGCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.


Preparation Note

Following initial thaw, aliquot and freeze (-70°C).


Other Notes

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
Waltregny, D., et al. 2005. FASEB J.19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
recombinantexpressed in insect cells (S. frugiperda)
Quality Segment100
assay≥90% (SDS-PAGE)
formliquid
specific activity≥10 units/μg protein
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
shipped inwet ice
storage temp.−70°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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