- 商品介绍
- 规格参数
- 包装参数
General description
25.24 kDa calculated.
T-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
Immunogen
EL-4 T lymphoma cells.
Application
Affects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. al. (2007) Blood. 1;110(7):2528-36).
Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).
Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).
Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8
This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.
Biochem/physiol Actions
Clone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells.
Physical form
Format: Purified
Purified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Analysis Note
Evaluated by Flow Cytometry in EL-4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | hamster (Syrian) |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 37.51, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_031668.3 |
| UniProt accession no. | P31041 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd28(12487) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 1148.92
-
询价
-
询价
-
¥ 5887.85
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 954.17
-
¥ 2173.94
-
¥ 2911.61
-
¥ 3662.39
-
询价
-
询价
-
¥ 165230.53
-
¥ 17652.29
-
询价
-
¥ 108621.02
-
¥ 52185.37
微信小程序
7X24小时在线咨询