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General description
83 kDa
Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
Immunogen
Synthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
Application
Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.
Immunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation.
ELISA: Recommended.
Optimal working dilutions must be determined by end user.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Biochem/physiol Actions
Recognizes p90 RSK1.
Physical form
Purified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.
Preparation Note
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Analysis Note
Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-417
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, mouse, human |
| technique(s) | ELISA: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_001006665.1 |
| UniProt accession no. | Q15418 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RPS6KA1(6195) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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