Anti-trimethyl Histone H3 (Lys79) Antibody, Trial Size,10UG,07-952-S,Sigma

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订货号 0BV5104
品牌型号 Sigma 07-952-S
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

~ 17 kDa


Immunogen

Epitope: Lys79
Linear Peptide corresponding to human Histone H3 methylated at Lys79.


Application


Dot Blot Specificity Analysis:
AbSurance Histone H3 Antibody Specificity Arrays (Cat. #: 16-667 and 16-665) containing peptides to all key histone modification sites, were probed with Anti-trimethyl Histone H3 (Lys79) Antibody Antibody ; dilution 1:5000. Proteins were visualized using a goat anti-mouse IgG (H+L) conjugated to HRP and a chemiluminescence detection system.


Multiplex Immunoassay
Specificity of interaction of anti-trimethyl histone H3 Lys79 antibody was assayed using the Luminex multiplex immunoassay platform. Anti-trimethyl-Histone H3 (Lys79) diluted to 0.01 mg/ml concentration was incubated with a cocktail of xMAP microspheres conjugated to the following histone H3 peptides, monomethyl Lys79, unmodified Lys79, trimethyl Lys 27, trimethyl Lys 9, trimethyl Lys79 processed and detected using standard xMAP protocols.
Anti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79) also known as H3K79me2, Histone H3 (di methyl K79) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology


Biochem/physiol Actions

Broad species reactivity is expected.
This antibody recognizes Histone H3 methylated at Lys79.


Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.


Preparation Note

Stable for 1 year at 2-8°C from date of receipt.


Analysis Note

Control
HeLa acid extract lysate and recombinant Histone H3
Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.


Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formaffinity isolated antibody
antibody product typeprimary antibodies
clonepolyclonal
purified byaffinity chromatography
species reactivityhuman
technique(s)dot blot: suitable,multiplexing: suitable,western blot: suitable
NCBI accession no.NP_003484.1
UniProt accession no.Q16695
shipped inwet ice
target post-translational modificationtrimethylation (Lys79)
Gene Informationhuman ... H3C1(8350)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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