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General description
21 kDa calculated.
Interferon alpha-2 (UniProt P01563; also known as IFN-alpha-2, Interferon alpha-A, LeIF A) is encoded by the IFNA2 (also known as IFNA2A, IFNA2B, IFNA2C) gene (Gene ID 3440) in human. Interferons (IFNs) are cytokines whose prouction and release by host cells are induced in the presence of pathogens (e.g. viruses, bacteria, parasites) and tumor cells. Originally named for their ability to interfere with viral replication, IFNs mediate the activation of immune cells, such as natural killer cells and macrophages, and increase host defense by up-regulating antigen presentation by boosting the expression of major histocompatibility complex (MHC) antigens. More than twenty distinct IFN genes and proteins have been identified and classified into three main types. Type I IFNs (IFN-α, IFN-β, IFN-ε, IFN-κ, and IFN-ω) exert their function via binding IFN-α/β receptor (encoded by the IFNAR1 and IFNAR2 gene, respectively). IFN-γ is the only known type II IFN, it is released by type 1 T helper cells and binds IFN-γ receptor (encoded by the IFNGR1 and IFNGR2 gene, respectively). Type III IFNs signal through a receptor complex consisting of IL10R2 (CRF2-4) and IFNLR1 (CRF2-12).
This Anti-Interferon-α2 Antibody, clone ST254 (Ascites Free) is validated for use in Western Blotting, Affinity Binding Assays, ELISA and Neutralizing.
Immunogen
Human alpha-interferon.
Application
Neutralizing Analysis: A representative lot enhanced viral replication by neutralizing host-secreted IFN in human prostate cancer PC3 cultures infected with P/V-CPI(−) and P/V-CPI(−)-G3A at a low MOI (Gainey, M.D., et al. (2000). J. Virol. 82(19):9369-9380).
Neutralizing Analysis: A representative lot neutralized human IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells), but not IFN-α1, antiviral activity against Semliki Forest Virus in human WISH amniotic epithelial cell cultures (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Affinity Binding Assay: A representative lot captured human IFN-α2, but not human IFN-α1 (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
ELISA Analysis: A representative lot was used as the detection antibody for the detection of human IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells) by sandwich ELISA (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Western Blotting Analysis: A representative lot detected IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells) under reducing condition by Western blotting (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Interferon-α2 Antibody, clone ST254 is validated for use in Western Blotting, Affinity Binding Assays, ELISA and Neutralizing
Biochem/physiol Actions
Clone ST254 neutralized human IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells), but not IFN-α1, antiviral activity (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Polyacrylamide Gel Electrophoresis.
Purity of this monoclonal antibody is determined to be at least >85% by SDS-PAGE.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | ST254, monoclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,neutralization: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000596 |
| UniProt accession no. | P01563 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... IFNA2(3440) |
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