首页> 生物试剂> 其他生物试剂> 其他> Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells，10UG，219467-10UGCN，Sigma

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Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells，10UG，219467-10UGCN，Sigma

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订货号： 0BV5517

品牌型号： Sigma 219467-10UGCN

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最小订货量： 1件

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商品介绍
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包装参数

产品介绍 Product Description

General description

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.

Preparation Note

To activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
Tolosa, E., et al. 2003. J. Clin. Invest.112, 517.
Turk, V., et al. 2001. EMBO J.20, 4629.
Somoza, J.R., et al. 2000. Biochemistry39, 12543.
Bromme, D., et al. 1999. Biochemistry38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci.39, 1789.
Santamaria, I., et al. 1998. Cancer Res.58, 1624.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

recombinant	expressed in NSO cells
Quality Segment	100
assay	≥95% (SDS-PAGE)
form	frozen liquid
specific activity	≥300 units/μg
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
impurities	≤1.0 EU/μg Endotoxins (EU/μg Cathepsin V)
shipped in	wet ice
storage temp.	−70°C

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