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- 规格参数
- 包装参数
General description
Tumor Necrosis Factor-alpha (TNF-alpha), also known as TSG-6, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. TNF-alpha induced protein 6 may be involved in cell-cell and cell-matrix interactions during normal inflammatory responses and during tumorigenesis.
~29 kDa observed.
An uncharacterized band appears at ~50 kDa in some lysates.
Immunogen
Epitope: Unknown
GST-tagged recombinant protein corresponding to mouse TSG-6.
Application
Detect TNF-alpha induced protein 6 (TSG-6) using this Anti-TNF alpha Antibody, clone NG3 validated for use in WB, ELISA. This TNF alpha antibody has been tested in Human and Mouse.
ELISA: A previous lot of this antibody was used in capture ELISA to demonstrate a typical standard curve for measuring TSG6 from normal (naïve) serum.
Western Blot Analysis: A previous lot of this antibody was show to detect human or mouse samples (Nagyeri, G., et al. (2011). JBC. PMID: 21566135).
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Biochem/physiol Actions
Published to react with human TSG-6 (Nagyeri, G., et al. (2011). JBC. PMID: 21566135).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse ovary tissue lysate
Evaluated by Western Blot in mouse ovary tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected TSG-6 in 10 µg of mouse ovary tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | NG3, monoclonal |
| species reactivity | human, mouse |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_033424 |
| UniProt accession no. | O08859 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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